Raw material Propolis Extract powder

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Propolis Extract powder Usage and Synthesis.

Propolis extract is a yellow-brown or black-brown viscous material extract secreted by the Chinese honeybee or Italian bee, an animal of the honeybee family of the Apis mellifera, the main components of which are flavonoids and terpenes, etc. It has the functions of antimicrobial, anti-inflammatory, viral inhibition, and enhancement of immunity.

Propolis is a gelatinous substance synthesised by bees through repeated metabolism of the resin collected from the buds and bark of long-glue plants, mixed with secretions from glands such as the tongue and wax glands of bees.

Propolis is a reddish-brown to greenish-brown powder, or resin-like lumps, with a fragrance. When heated, there are waxes, can be dispersed in water, and also has the role of surfactant.

Uses of Propolis Extract powder.

Firstly, propolis is rich in flavonoids, which can reduce the serum lipid peroxide content of diabetic rats and enhance the vitality of glutathione peroxidase, thus inhibiting blood glucose and preventing and treating diabetes.

Secondly, the flavonoids and terpenoids in propolis extract can promote the synthesis of hepatic glycogen using exogenous glucose and lower blood glucose. In addition, propolis extract has antioxidant and hypoglycaemic effects.

Components such as quercetin and rutin in propolis also have the effects of coronary vasodilatation, lipid lowering, antiplatelet aggregation, anti-inflammatory, antiviral, and antitumour effects. Therefore, propolis extracts can be applied to the prevention and treatment of various diseases such as diabetes, cardiovascular and cerebrovascular diseases, and tumours.

Pharmacological effect of Propolis Extract powder.

1.Antibacterial effect

Propolis alcohol extract 35-750μg/ml in vitro test, the inhibitory effect on Staphylococcus aureus, can produce resistance, but with the general antibiotics, no cross-resistance found.

2.Antifungal effect

Propolis alcohol infusion or ether infusion 1% to 10% concentration, with plate paper disc method test, found to have inhibitory effect on ringworm, flocculent epidermal ringworm, red ringworm, rust-coloured microsporidium, woolly microsporidium, gypsum-like ringworm, gypsum-like microsporidium, hair-breaking ringworm and so on.

3.Antiprotozoal

Propolis ethanol extract 0.5～150μg/ml in vitro test, has the effect of killing Trichomonas vaginalis.

4. Anti-cancer effect

Propolis ethanol extract has inhibitory effect on HeLa cells in vitro test, IC50 is 10μg/ml.

5.Anti-radiation damage in rats pre-administered propolis extract 20mg, half an hour before irradiation, can counteract the decline in leukocyte alkaline phosphatase activity induced by X-ray irradiation.

6.Antioxidant effect

Propolis ethanol extract at 0.04% concentration significantly increased the stability of porcine lipids. In mice infected with S. typhimurium, superoxide dismutase (SOD) activity in small intestine homogenate decreased, lipid peroxidation increased, DNA enzyme and histone D activity increased, and propolis 2mg given 1h after S. typhimurium infection could reduce these pathological changes.

Excessive intake of polyunsaturated fatty acids in rats increased free radicals, lipid oxidation and decreased antioxidant levels in vivo, and ingestion of vitamin E or propolis 20mg for 20d prevented these changes, and the antioxidant effect of propolis was also more pronounced than that of vitamin E. Propolis increased the activities of hepatic superoxide dismutase, glutathione peroxidase, reduced glutathione, DNA enzyme, RNA enzyme, and histone D activity, as well as the hepatic protein levels. and hepatic protein levels.

Preparation method of Bulk Propolis Extract powder.

1. Dichloromethane method takes out the propolis after refrigerating and crushing, adds a certain amount of dichloromethane, stirs thoroughly, carries on the heating reflux, sets aside for a few hours, carries on the filtration, then lets the solvent volatilise, then extracts with the ethanol hot reflux, filtration, removes the wax, can obtain the propolis extract.

2. Ethyl acetate method according to the principle of similar solubility of polar molecules, the use of ethyl acetate to dissolve propolis, the formation of dip.

The total amount of flavonoids can reach 22.5%. 3. Boron polymer electrolyte method This method is the latest research results of Japanese scientists, specifically the anionic polymer compounds - boron polymer electrolyte diluted refined aqueous solution with the cellulose containing propolis fully miscible with the polymer electrolyte diluted refined aqueous solution can be continuously decomposed The total amount of flavonoids can reach 22.5%.

3. boron polymer electrolyte method of this method is the latest research results of Japanese scientists, specifically the anionic polymer compounds - boron polymer electrolyte diluted refined aqueous solution with the cellulose containing propolis fully miscible polymer electrolyte diluted refined aqueous solution can be constantly decomposed propolis, and then add ethanol and to algae By adding ethanol and cellulose made from seaweed, a hydrophilic gel containing the active ingredient can be extracted, and the crystals obtained after refining have strong antibacterial power, and the extract obtained by this method has good activity.

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