Cholic Acid Powder CAS 81-25-4 Raw Material

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Cholic Acid Powder Usage and Synthesis.

Cholic Acid Powder is a host and microbiota related metabolite, Cholic Acid Powder is a colorless flake or white crystalline powder that modulates host Cholic Acid CAS 81-25-4 is synthesized in the host and secreted into the gut where microorganisms can further Cholic Acid Raw Material is used in biochemical research, pharmaceutical intermediates. Sodium cholic acid is a cholagogue and treats cholecystitis, bile deficiency, and intestinal dyspepsia.

Application/Function of Cholic Acid Powder.

1. Cholic Acid Powder is used in biochemical research, pharmaceutical intermediates. Sodium cholic acid is a cholagogue, treating cholecystitis, bile deficiency, and intestinal dyspepsia.
2. Cholic Acid Powder is a non-denaturing ionic detergent used to extract membrane proteins
3. Cholic Acid Powder is a non-denaturing ionic detergent for the extraction of membrane proteins.

Production method of Cholic Acid Powder

• Method I. Preparation of crude bovine and sheep bile acid by ethanol crystallization method Take bovine or sheep bile, add 100g/L sodium hydroxide, heat and boil for 12-18h to obtain saponification solution. After cooling, add acid and adjust pH1, precipitate bile acid, remove the bile acid, boil, rinse, dry at 75℃, grind, and obtain crude bovine and sheep bile acid.
• NaOH]→[100℃,12-18h]saponification solution[H2SO4]→[pH1,75℃]crude bile acid of bovine and sheep Preparation of finished bile acid of bovine and sheep Take crude bile acid, add 0.5-1 times the amount of 95% ethanol, heat reflux until the solid dissolves, cool. Remove the crystals and mash, filter, add 95% ethanol and wash until the filtrate is colorless. Add 4 times the amount of ethanol to the crystals, add 100-150g/L activated carbon, heat reflux until the crystals dissolve, filter while hot, concentrate the filtrate to 1/4 of the original volume, cool, crystallize, filter, add ethanol to wash the crystals, dry, and get the finished product of bile acid of cattle and sheep. Crude taurocholic acid [ethanol, activated carbon] → refined solution [below 90℃] → finished taurocholic acid.

• Method 2, ethyl acetate separation method for the preparation of crude pig bile acid take fresh pig bile, add 3-3.5 times the amount of saturated lime water supernatant under stirring, to be added and continue to stir 5-10min, heated to boiling 2min, cooling, filtration, filtrate with hydrochloric acid pH 3.5, precipitation, rest for more than 12h, to obtain crude bile acid. Take out, wash with water, add 1.5 times of sodium hydroxide, add 9 times of water, heat and boil for 12-18h, let cool, let stand overnight, get paste. Add water, add sulfuric acid to adjust pH1, precipitate pig bile acid. Take out, mash, rinse until no acid, filter, and get crude pig bile acid.
• Pig bile juice [saturated lime water] → [100℃, pH11-12] alkaline filtrate [HCl] → [pH3.5] crude bile acid [water, NaOH] → paste [H2SO4] → [pH1] Preparation of crude pig bile acid Pig bile acid finished products take crude pig bile acid, add 4 times the amount of ethyl acetate, add 150-200g/L activated carbon, heat reflux for 0.5h, let cool, filter, filter cake Add 1.5-2.5 times of ethyl acetate for 1 time, and combine the two filtrates. Add 200g/L anhydrous sodium sulfate, leave it overnight, concentrate to 1/3 of the original volume, release, cool down and crystallize, filter, wash the crystals with ethyl acetate, dry, and get the finished product of porcine bile acid. Crude porcine bile acid [ethyl acetate, activated carbon] → filtrate [anhydrous sodium sulfate] → filtrate [concentrate] → finished porcine bile acid.

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