cellulase Raw Materials powder cellulase

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cellulase powder Usage and Synthesis.

Cellulase is now widely used in various fields such as textile, daily chemicals, paper making, food fermentation, industrial washing, tobacco, oil extraction, wastewater treatment and feed, etc.and its application prospect is very broad. Natural cellulase exists in microorganisms, certain insects and plants.

When plant seeds germinate, cellulase hydrolyzes seed coat cellulose, facilitating seedling emergence. Symbiotic bacteria and protozoa in the digestive tract of herbivorous ruminants can secrete cellulase, which hydrolyzes cellulose into absorbable sugars.

The use of microbial secretion of cellulase for agricultural by-product processing, can produce high-quality feed and glucose. Agricultural application of green wood mold (a fungus rich in cellulase) for feed fermentation, can improve the nutritional value of cellulose feed.

Cellulase products are one of the main reagents for separating plant protoplasts, and in plant cell fusion experiments, cellulase or snail enzyme is commonly used to break down and remove the cell wall in order to separate and obtain the required protoplasts.

Most industrial cellulases are glucoside endonucleases containing a catalytic region and a cellulose linkage region. Without the cellulose linkage region the enzyme has low activity for cellulose. A controlled study of cellulases and proteases has been carried out and found that the activity of cellulases is more related to the strength of adsorption.

Amino acid residue Tyr at position 169 in the catalytic region of the cellobiose hydrolase of Trichodermareesei assists in the conversion of the glucose ring to a more reactive conformation.

Uses of cellulase powder.

Cellulase is mainly used for softening and peeling of grains, beans and other plant foods; controlling (lowering) the viscosity of coffee extracts, with a maximum permissible dosage of 100mg/kg; pre-treatment of brewing raw materials; manufacture of starch, agar and seaweed foods; elimination of turbidity in fruit juices caused by cellulose species; and instant solubilization of green tea, black tea and so on. As a feed additive, it helps animals to digest and absorb the feed.

Used in biochemical research, plant cell hybridization research, used to catalyze the hydrolysis of cellulose molecules for oligocellulose and enzymes, medicine is commonly used as a digestive enzyme.

1、Enzyme preparation.2、Mainly used for softening and peeling of cereals, beans and other plant foods;

3, used to reduce the viscosity of coffee extract, the maximum allowable dosage of 100mg/kg; 4, used for brewing raw materials pretreatment; 5, used in the manufacture of skimmed soybean meal and separation of soy protein extraction; 6, used in the manufacture of starch, agar and seaweed food; 7, to eliminate the turbidity of fruit juices, wines, beers and so on, caused by fibers; green tea, black tea, such as the instantaneous solubilization

Characteristic of cellulase powder.

Off-white amorphous powder or liquid. Soluble in water, almost insoluble in ethanol, chloroform and ether. It is more stable to heat, even if it is kept at 100℃ for 10min, it can still keep 20% of the original activity. General optimum temperature for the role of 50 ~ 60 ℃, the optimum Ph value of 4.5 ~ 5.5. by Aspergillus niger and Aspergillus platensis extracted by the product ADI does not make special provisions for the production of ADI by Penicillium unspecified (FAO/WHO, 1994).

Product Method of Bulk cellulase powder.

Generally, Aspergillus niger (Aspergillusniger) or Trichodermareesei (T. longibrachiatum) is used for cultivation, and then the fermentation broth is precipitated and refined by salting out. In addition to cellulase, the resulting product also contains hemicellulase, pectinase, protease, lipase, xylanase, cellulase and starch glucosidase.

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