Organic Syntheses Material Intermediate Chlorophyll powder

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Chlorophyll powder Usage and Synthesis.

Chlorophyll is a natural pigment found in plants and has important biological functions.

Total chlorophyll is the total amount of all chlorophyll in plant leaves, which can be extracted and measured to understand the growth status and nutritional level of plants. At present, there are two main methods to extract total chlorophyll: extraction by organic solvents and separation by physical methods.

In addition, chlorophyll is also widely used in the food processing industry, which is a pigment raw material with nutritional and health care functions. In the field of agriculture, chlorophyll tester can be used for soil and water quality monitoring and plant nutrition diagnosis.

Uses of Chlorophyll powder.

Chlorophyll is used for colouring soaps, mineral oils, waxes and essential oils. Chlorophyll or chlorophyllic acid derivatives, e.g. copper chlorophyll, sodium iron chlorophyllate, sodium copper chlorophyllate, are used as colouring and deodorising agents in foods, confectionery, beverages, toothpaste, etc. Chlorophyllic acid derivatives can be used in conjunction with fungicides such as Jell-O and Halocarban as formulas for odour cosmetics.

Chlorophyll is suitable for colouring or complementary colouring of prepared wine, green juice, fruit wine, vegetable juice, chewing gum, toothpaste, filled snacks and high-grade candies.

Characteristic of Chlorophyll powder.

Chlorophyll a: Magnesiumcomplexsaltof1,3,5,8-tetramethyl-4-ethyl-2-vinyl-9-oxo-10carbome-thoxyphorbin-7-propionicacidphytylester,R=CH3, is a waxy Blue-black fine crystals.

Often sword-like or blade-like. Soluble in ether, alcohol, acetone, chloroform, carbon disulfide and benzene, insoluble in cold methanol, almost insoluble in petroleum ether. Its alcohol solution is blue-green and shows deep red fluorescence.

Melting point 117-120°C (150-153°C, decomposition). Spinning luminosity -262°C (chloroform). Maximum absorption value (ether) 660, 613, 557, 531, 498, 429, 409nm.

Product Method of Bulk Chlorophyll powder.

Chlorophyll is mostly extracted from plants (e.g. spinach, etc.) or dried silkworm sand. For example, the following methods can be used to extract chlorophyll from silkworm sand.

Organic solvent extraction: take clean silkworm sand, with more than 70% of industrial ethanol into a slurry, filter out the dark green solution, and then dried and obtained. Another operation is 45 parts of petroleum ether, 15 parts of methanol, 4 parts of benzene mixed with clean silkworm sand mixed slurry, filtration, filtrate washed with water 4 times.

In the organic extract add a small amount of sodium sulfate to remove residual water, filtration, filtrate recovery of solvents after the chlorophyll. Physical method of separation: 0.4 mol / L dilute sucrose solution and 0.06 mol / L potassium phosphate dilute solution (pH 6-7) according to 1:1 mixed into a buffer medium.

For every 2kg of silkworm sand, add about 1L of buffer medium, mix well, filter out the green suspension with multi-layer sandcloth, put it into a low-temperature centrifuge and centrifuge for 5-10min, wash the precipitate with water, and centrifuge again to get the chlorophyll precipitates.

Chlorophyll was treated with oxalic acid to obtain magnesium-free demagnesium chlorophyll, and then magnesium was introduced and converted back to chlorophyll. Removal of phytol and magnesium by strong acid gives demagnesium chlorophyll, and phytol can be reintroduced. Hydrolysis removes phytol and methanol and gives chlorophyll.

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