Supplement Turkestanica Extract raw Turkesterone Powder

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Turkesterone Powder Usage and Synthesis

China Turkesterone Raw Material Factory Supply Turkesterone Raw Material, High Quality Turkesterone Powder Purity 99% Turkesterone Raw Powder, Turkesterone Powder is isolated from Ajuga turkestanica, Turkesterone is a potent ecdysteroid.Manufactory Supply Turkesterone Raw Material, High Purity Turkesterone Powder, High Quality Turkesterone Raw Powder.

Dew grass (CyanotisarachnoideaC.B.Clarke), also known as pearl dew grass, cocklebur ginseng, etc., the Department of duckweed family orchid ear grass perennial herbaceous plants, widely distributed in Yunnan Province, with the effect of tonifying the deficiency, removing dampness, soothing the tendons and activating the collaterals, the first time to isolate the Turkestanone from the plant.

Uses and functions of Turkesterone Powder

Turkesterone was isolated from Ajuga turkestanica, a potent ecdysteroid used as an ecdysteroid receptor (EcR) agonist in some insect systems.

Bioactive pharmacological effects of Turkesterone Powder

Dew grass (2.5kg) was extracted with 75% ethanol by heating and refluxing, recovering the ethanol and obtaining the concentrate. Extracted with ethyl acetate, n-butanol;

The ethyl acetate extract was taken, and then chromatographed on an Al2O3 (neutral) column with a gradient elution of chloroform-methanol, and the chloroform-methanol (4:1) eluted portion was recrystallised from ethyl acetate-methanol to obtain compound 3 (200mg);

The 3:1 eluted portion was subjected to preparative thin layer chromatography (stationary phase: silica gel GF254;

Mobile phase: chloroform-methanol=5:1) to obtain compound 1 (10mg);

The methanol eluted portion was separated by preparative high performance liquid chromatography (ODS, methanol/water=31:69, detection at 242 nm) to obtain Turkestanone (5 mg).

Production method of Turkesterone Powder

• Dew grass (2.5kg) was extracted with 75% ethanol by heating and refluxing, recovering the ethanol and obtaining the concentrate. Extracted with ethyl acetate, n-butanol;
• The ethyl acetate extract was taken, and then chromatographed on an Al2O3 (neutral) column with a gradient elution of chloroform-methanol, and the chloroform-methanol (4:1) eluted portion was recrystallised from ethyl acetate-methanol to obtain compound 3 (200mg);
• The 3:1 eluted portion was subjected to preparative thin layer chromatography (stationary phase: silica gel GF254; mobile phase: chloroform-methanol=5:1) to obtain compound 1 (10 mg);
• The methanol eluted portion was separated by preparative high performance liquid chromatography (ODS, methanol/water=31:69, detection at 242 nm) to obtain Turkestanone (5 mg).

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