Cellulase Powder CAS 9012-54-8 Raw Materials

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Cellulase Powder Usage and Synthesis.

Cellulase Powder is the general name of a group of enzymes that catalyze the hydrolysis of cellulose to produce glucose and low-polymerization fibers. Cellulase Powder is the general name of a group of enzymes that catalyze the hydrolysis of cellulose to produce glucose and low-polymerization fibers, including three main components: glucan endonuclease, glucan exonuclease and cellobiase, which is not a single enzyme but a multi-component enzyme Cellulase CAS CAS:9012-54-8

Cellulase CAS 9012-54-8 is used for the treatment of indigestion and loss of appetite. It is used in biochemical research, plant cell hybridization It is used in biochemical research, plant cell hybridization research, to catalyze the hydrolysis of cellulose molecules into oligomeric cellulose and enzymes, and is commonly used as a digestive enzyme in It is commonly used as a digestive enzyme in medicine.

Source of Cellulase Powder.

Cellulase can be produced by insects, mollusks, protozoa, bacteria, actinomycetes, fungi, etc.

• 1. Bacteria: The amount of cellulase produced by bacteria is low, mainly EG, most of them have no activity on crystalline cellulose, and the enzymes produced are intracellular enzymes or adsorbed on the bacterial wall, rarely can be secreted outside the cell, which increases the difficulty of extraction and purification, and are seldom used in industry in practice. At present, more research is cellulose mucilage genus, spore-bearing fiber mucilage genus and fiber bacillus genus.
• 2. Actinomycetes: little research on actinomycetes. Bifidobacteria and proto-actinomycetes hardly produce cellulase or the yield is very low, and the main ones with slightly higher yield are black and red spiral actinomycetes, rose-colored actinomycetes, fiber actinomycetes and white rose actinomycetes.

• 3. fungi: most of the microorganisms used in the production of cellulase are fungi, more research on the genus Xylella, Aspergillus, Rhizopus and Lacertus. They can produce a large number of cellulase, especially in the genus Xylem production. Lee's Xylella and green Xylella is the genus Xylella in the enzyme activity of the higher species. Fungi produce three types of cellulase, can be secreted into the fungus, generally do not gather to form a multi-enzyme complex, but strong synergistic effect occurs with each other. Currently have been made into preparations of green mold, Aspergillus niger, Fusarium, as well as Penicillium and Penicillium obliquum cellulase.
• 4. yeast: due to the need for industrial production of cellulase, the requirement to improve the enzyme yield, exocytosis and heat resistance. Recently, there has been more research on cellulase gene cloning. Although almost all the cloned genes can be expressed in E. coli, but due to the difficulty of extraction and low expression level, the research on gene expression is now shifted to eukaryotic expression system, which is currently more applied to yeast expression system.

Yeast does not produce toxins, and the cellulase genes expressed with it are highly glycosylated and can be secreted directly into the medium with high expression levels after correct processing and modification. For example, the yield of CHBⅡ and EGⅠ expressed by yeast can reach more than 100mg/L and have normal biological activity.

Application/Function of Cellulase Powder.

• 1、Enzyme preparation.
• 2、Mainly used for softening and peeling of cereals, beans and other plant foods;
• 3、Used to reduce the viscosity of coffee extracts, the maximum allowable dosage is 100mg/kg;
• 4、Used for pretreatment of brewing raw materials;
• 5, for extraction in the manufacture of defatted soybean powder and isolated soy protein;
• 6, used in the manufacture of starch, agar and seaweed food;
• 7、Eliminate the turbidity caused by fiber type in juice, wine, beer, etc.; instant dissolution of green tea, black tea, etc.

Cellulase is now widely used in various fields such as textile, daily chemical, papermaking, food fermentation, industrial washing, tobacco, petroleum extraction, waste water treatment and feed, etc. Its application prospect is very broad.

Natural cellulase is found in microorganisms, some insects and plants. When plant seeds germinate, cellulase hydrolyzes seed coat cellulose to facilitate seedling emergence. Symbiotic bacteria and protozoa in the digestive tract of herbivorous ruminants secrete cellulase, which hydrolyzes cellulose into absorbable sugars. The use of microbial secretion of cellulase for agricultural by-products processing, can produce high-quality feed and glucose.

Agricultural application of green wood mold (a fungus rich in cellulase) for feed fermentation, can improve the nutritional value of cellulosic feed.

Cellulase products are one of the main reagents for separating plant protoplasts. In plant cell fusion experiments, cellulase or snailase is commonly used to break down and remove the cell wall to separate and obtain the required protoplasts.

Most industrial cellulases are glucosidic endonucleases, containing a catalytic region and a cellulose ligand region.

Without a cellulose linker region the enzyme has low activity for cellulose. A controlled study of cellulases and proteases found that the activity of cellulases was more related to the strength of adsorption.

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