Anthelmintics SPINOSAD Raw Materials powder

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Product details

SPINOSAD of Usage and Synthesis.

By binding with nicotinic acetylcholine receptors, polycidin depolarizates insect nerve cells, causing extensive hyperactivation of the central nervous system, leading to non-functional muscle contraction and failure, accompanied by trembling and paralysis, and having rapid contact and ingestion toxicity to insects. At the same time, it also hyperactivates nerve cells by inhibiting Y-aminobutyrate receptors, further enhancing their activity. However, the application site is different from that of nicotine or imidacloprid. Cause paralysis of the system by touching or eating. The effect is seen on the same day after spraying, and the insecticidal speed can be comparable to that of chemical pesticides, and non-ordinary biological insecticides are comparable.

Uses of SPINOSAD.

A new type of biological insecticide, with a unique chemical structure and the characteristics of safety and rapid effect, has been low toxicity, low residue, safety and other characteristics, won the United States "President's Green Chemical Challenge Award". It works by stimulating the insect's nervous system, causing non-functional muscle contraction and failure, accompanied by tremors and paralysis.

The results are consistent with the activation of nicotinic acetylcholine receptor, in addition, it also acts on γ-aminobutyric acid receptor, further promoting its insecticidal activity. This mode of action is favored. Polycidin can effectively control lepidoptera (such as diamonds, beet butterflies, etc.), diptera and thysanoptera pests, and can also control some insect species in Coleoptera and orthoptera that eat a lot of leaves. It is not effective against stinging insects and mites.

It is used to control lepidoptera larvae, thrips and leaf miner flies, etc., and has good control effect on cabbage moth, beetworm moth, leaf miner fly, thrips, Calliope moth and pod moth.

Drug interaction of SPINOSAD.

Product method of Bulk SPINOSAD Powder.

The new microbe Saccharo-polyspora spinosa ora mutant strain capable of producing polycidin. Air mycelium was produced on composite medium and synthetic medium. The color of the air spores is mainly pale yellowish-pink, but also white, and yellow to yellowish-brown on the opposite side, without obvious pigmentation.

The seed media for the fermentation culture of polycidin polyspora were: soybean hydrolysate 3%, yeast paste 0.3%, MgSO4·H2O 0.2%, glucose 0.5%, maltose.4%, deionized water 1L, sterilized at 121℃ for 30min. The dose is 10%. In large-scale production, the preferred carbon sources are glucose and maltose, but also ribose, xylose, fructose, galactose, mannose, mannitol, soluble starch, potato dextrin, methyl oleate, oil, etc. The preferred nitrogen is raffinose, peptonized milk and digested soybean powder. Fish meal, corn infusion, yeast paste, hydrolyzed casein and beef paste can also be used. The inorganic salts incorporated into the medium have conventional soluble salts that can produce the following ions: zinc ions, sodium ions, magnesium ions, ammonium ions, chloride ions, carbonate ions, sulfate ions, nitrate ions, etc. In addition, the medium should also contain the necessary trace elements required for the growth and development of microorganisms. If foaming is a problem, soybean oil can be added to the medium to inhibit foaming. The culture temperature of polyspora polycidin was 24 ~ 33℃, and the optimum temperature of polycidin production was 28 ~ 30℃. During industrial fermentation, the dissolved oxygen in the tank should be maintained at more than 60%, preferably more than 65%, by ventilation and stirring speed. The pressure inside the tank is 0.034MPa.

After fermentation for 7 ~ 10 days, the same volume of acetone was added, the bacteria were removed by filtration, the pH value of the filtrate was adjusted to 13, and the resin was adsorbed. Polycidin A and D were gradient eluted with a solution of 0 ~ 95% methanol: acetonitrilene = 1:1 (containing 0.1% sodium acetate), and the eluents were collected in stages to obtain polycidin eluents, which were concentrated to obtain polycidin concentrate. The concentrated solution was diluted with petroleum ether, on A silica gel column, gradient elution with petroleum ether and methanol, and then collected in stages to obtain dicidin A and D elutes.

Analysis and detection of high performance liquid chromatograph (HPLC). Column: ODS-AO, liquid phase: acetonitrile/methanol/water =40/40/20 (containing 0.05% ammonium acetate), flow rate: 3mL/min, detection wavelength: 250nm.

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