Natural Phaseolus Vulgaris Extract powder

CAS:85085-22-9

MF:/

MW:/

EINECS:285-354-6

Specification​: 99% min Phaseolus Vulgaris Extract powder

Sample:Phaseolus Vulgaris Extract powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance: White

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Phaseolus Vulgaris Extract powder Usage and Synthesis.

White Kidney Bean Extract is the mature seed extract of Phaseolusvulgaris, a herbaceous vine plant of the legume family;

It mainly contains proteins, fats, carbohydrates and some highly active functional substances such as phytohaemagglutinin (PHA), α-amylase inhibitors, polysaccharides and dietary fibre, flavonoids, phytohemagglutinins, food colouring, etc. and some nutrients such as vitamins, minerals, and amino acids such as lysine, leucine, and arginine, and potassium, magnesium, and sodium;

Among them, insoluble dietary fibre has the effect of reducing the probability of developing intestinal cancer, water-soluble dietary fibre has the function of adjusting the metabolism of sugars and lipids, flavonoids have the functions of antibacterial, anti-inflammatory, anti-mutagenic, antihypertensive, purging and detoxification, improving microcirculation, anti-tumor, etc. Kidney bean pigments have better light and heat stability and crystallinity, etc., amylase inhibitor has the effect of lowering blood sugar, trypsin inhibitor and protein have the effect on the tumour cell development inhibition; commonly used in health food raw materials in biological products.

Uses and functions of Phaseolus Vulgaris Extract powder.

1. As a source of raw materials for the production of white kidney bean peptides and amino acids.

2. Used in health food raw materials for biological products, as a high-potassium and low-sodium food for patients with high blood lipids, heart disease, arteriosclerosis and salt avoidance.

3. White kidney bean protein contains a natural α-amylase inhibitor, which can be used in the treatment of obesity, hyperlipidaemia, arteriosclerosis, hyperlipidaemia and diabetes.

4. It is used for haemostasis and animal genetic analysis.

Function of Phaseolus Vulgaris Extract powder.

1. polysaccharides and dietary fibre dietary fibre is mainly two kinds of dietary fibre, of which insoluble dietary fibre can absorb water, soften the stool, increase the volume of stool, can stimulate intestinal peristalsis, accelerate defecation, in order to reduce the time of contact between harmful substances in the feces and the intestinal tract, and to reduce the probability of suffering from intestinal cancer; water-soluble dietary fibre has the function of adjusting the metabolism of sugars and lipids, and has good effects on lowering the human body's cholesterol level and preventing cardiovascular diseases. 2. It has good effect on lowering cholesterol level and preventing cardiovascular diseases.

2. Flavonoid bioflavonoids have various biological activities, such as antibacterial, anti-inflammatory, anti-mutagenic, antihypertensive, purging and detoxification, improving microcirculation, anti-tumour, anti-oxidation and other important functions.

3.PhytohaemagglutininPhytohaemagglutinin is a kind of glycoprotein mainly extracted and separated from plant seeds, which has important and special biological functions in plants and animals due to its specific binding of sugar, and has shown a very broad application prospect in the prevention and treatment of clinical diseases, the regulation of physiological activities of the body as well as bioengineering and other aspects.

4. Food colouring natural pigments exist in edible organisms (mainly in edible plants) and are extremely safe for consumption.

However, natural food colouring is generally difficult to crystallize, and light and heat stability is poor, limiting the application value. However, kidney bean pigment has better light and heat stability and crystallinity, and thus has a broad development prospect. The pigment can be added to food not only for colouring, but also for antioxidant and antibacterial effects.

5. Amylase inhibitor α-amylase inhibitor (α-amylaseinhibitor, α-AI) is a kind of glycoside hydrolase inhibitor. It inhibits the activity of salivary and pancreatic α-amylase in the intestinal tract, hinders the digestion and absorption of starch and other carbohydrates in food, selectively reduces sugar intake, lowers blood glucose levels, and reduces fat synthesis, thus playing a role in lowering glucose, losing weight and preventing obesity.

The α-AI extracted from white beans has high activity and strong inhibition of mammalian pancreatic α-amylase, which has been applied as weight loss health food abroad.

Extraction method of Bulk Phaseolus Vulgaris Extract powder.

1. Extraction of globulin: white kidney beans dried and crushed through a 60-mesh sieve, according to 1:10 to add distilled water, extracted at 40 ℃ for 2 times, each time 2h, centrifugation after extraction, discard the supernatant, take the precipitate dry, as the raw material for globulin extraction test. In the NaCl concentration of 2.5g/100mL, material-liquid ratio of 1:22 (g/mL), extraction temperature of 55 ℃, extraction time of 5h, etc. under the conditions of the globulin extract obtained by centrifugation at 4,000 rpm for 20min to take the supernatant, the determination of protein content in the supernatant.

2. Clear protein extraction: take 60 mesh fineness of white kidney bean powder, according to 1:8 (g/mL) of the material-liquid ratio by adding distilled water, at a temperature of 55 ℃ immersion 3h, 4000r/min centrifugation 20min, the supernatant is the white kidney bean clear protein extract.

3. Extraction of α-amylase inhibitor: There are two main methods for the preparation of α-amylase inhibitor, i.e., fermentation method and plant extraction method. The main steps of the isolation and purification methods for α-AI obtained as a plant source include grinding, heat treatment or extraction with water, precipitation with ammonium sulphate or separation with ethanol, separation after chromatography over DEAE-cellulose columns, gel chromatography over SephadexG-75 columns as well as chromatography over CM-cellulose columns, and lyophilisation to obtain the enzyme in the form of a white powder.

The purification method was also reported as follows: plant seeds were soaked in water → grinding of tissues → centrifugation, collection of supernatant → rapid warming, selective thermal denaturation → cooling to room temperature, centrifugation and collection of supernatant → precipitation by salting out → dialysis with buffer → DEAE-32 chromatography → concentration in polyethylene glycol 6000 → affinity chromatography → lyophilisation by concentration.