Theaflavin Raw Materials Powder CAS 4670-05-7

CAS:4670-05-7

MF:C29H24O12

MW:564.49

EINECS:/

Specification: 99% min Theaflavin Powder

Sample:Theaflavin Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance:Red

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Theaflavin Powder Usage and Synthesis.

Theaflavin is an important quality component and functional component of black tea, with hypolipidemic, hypotensive, antioxidant, antiradiation, antibacterial, anticancer and other biological activities and pharmacological efficacy, known as "tea in the soft gold", black tea in the the theaflavin content of only 0.5% -2.0%.

Theaflavin is a polyphenol oxidation of a class of substances can be dissolved in ethyl acetate, with the general name of the compound of benzopyranolone.

Theaflavin has 12 components, of which theaflavin (TF), theaflavin-3-gallate (TFMG), theaflavin-3, 3'-bis-gallate (TFdiG) and theaflavin-3'-gallate (TFM'G) is the four most important theaflavin (chemical). structures are shown above).

Theaflavin is purified as orange-yellow needle-like crystals with a melting point of 237-240°C. It is easily soluble in water, methanol, ethanol, acetone, n-butanol and ethyl acetate, and insoluble in ether. Insoluble in trichloromethane and benzene. Theaflavin solution is a distinct orange-yellow, aqueous solution is weakly acidic, pH about 5.7.

The color is not affected by the pH of the tea broth extract, but it has a tendency to auto-oxidize in alkaline solutions and is strengthened with the increase of pH.

Uses of Theaflavin.

Theaflavin is a compound present in black tea, which has a variety of beneficial effects and actions on the human body.

Firstly, after clinical trials, theaflavin has been proven to have the efficacy of regulating blood lipids and preventing cardiovascular diseases without side effects.

Secondly, theaflavins have a protective effect against myocardial damage.

In addition, theaflavins have antioxidant effects. In summary, theaflavins have important roles in regulating blood lipids, preventing cardiovascular diseases, protecting the myocardium and antioxidant.

Pharmacological effect of Theaflavin.

Theaflavin inhibited the proliferation and induced the apoptosis of human nasopharyngeal carcinoma CNE2 cells in a time- and concentration-dependent manner, and its mechanism of action may be related to the regulation of cell proliferation and apoptosis gene expression. Theaflavin can reduce serum uric acid in hyperuricemic mice.

The effects of theaflavins on serum uric acid were related to the inhibition of xanthine oxidase activity and the regulation of mRNA and protein expression levels of related anion transporters.

Theaflavin can enhance the sensitivity of cervical cancer cells to cisplatin, and its mechanism may be related to the increase in the expression of apoptosis-related proteins CleavedCaspase3, CleavedCaspase9 and PARP, and the inhibition of the expression of NF-κB signaling pathway-related proteins p-IκBα, p-NF-κB and p-Akt.

Theaflavins can effectively delay the inflammatory degeneration process of rat chondrocytes by enhancing the activity of chondrocyte synthesizing factors, attenuating the activity of catabolic factors and inhibiting the cellular inflammatory response, and have a certain protective effect on inflammatory chondrocytes.

Preparation of Bulk Theaflavin Powder.

Weighing 100g of tea crushed, add 1000ml deionized water (90 ℃) extraction for 30min, remove the extract; then add 500ml deionized water (90 ℃) extraction for 30min; combined extract, 300 mesh filter cloth filtration, 0.05um ceramic membrane treatment, the tea flavin microfiltration solution.

The theaflavin microfiltrate was pumped from the heavy-phase inlet into a two-stage tandem centrifugal extractor, and at the same time, ethyl acetate solution with 1/2 the volume of the theaflavin microfiltrate material was pumped, and the rotational speeds of the two tandem centrifugal extractors were all at 3000 rpm, the light-phase outlet solution (i.e., ester-phase solution) was collected, and then processed with low-temperature vacuum concentration at 40 ℃, and spray dried to obtain the theaflavin extracts, of which the purity of theaflavin was 21.35%.