Raw Materials Esculin powder

CAS:531-75-9

MF:C15H16O9

MW:340.28

EINECS:208-517-5

Specification​: 99% min Esculin Powder

Sample:Esculin Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance:Light White

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Esculin Powder Usage and Synthesis.

Qinpi Aesculin is a coumarin-like compound extracted from the dried branch bark or dry bark of the bitter Tochigi ash tree of the family Mulleinaceae, and is the active ingredient of the traditional Chinese medicine Qinpi, which is a white crystalline powder with a slightly bitter odour at room temperature.

Aesculin and its glycoside aesculetin are effective in the treatment of bacterial dysentery and bronchitis. In addition Qinpi Aesculetin also has anti-inflammatory, antibacterial, anticoagulant, analgesic and other activities, and has a significant diuretic effect on mice.

It can inhibit the aldose reductase of rat eye lens, and is the growth inhibitor of Bacillus subtilis, and also has inhibitory effect on chemical to cancer.

Uses of Esculin.

A coumarin glycoside with antioxidant activity and protection against chemically induced carcinogenesis. It is used in microbiology for the isolation and identification of enterococci (group D streptococci).

Qinpi methylin has anti-inflammatory, antibacterial, anticoagulant and analgesic activities, and has a significant diuretic effect in mice. It can inhibit the aldose reductase of rat eye lens, and is a growth inhibitor of Bacillus subtilis, and also has an inhibitory effect on chemical to cancer.

Differentiate of Esculin.

1. Chemical characterisation: take 1g of the powder, add 10mL of ethanol, reflux for 10min on a water bath and filter. Take 1mL of filtrate, add 2-3 drops of 1% ferric chloride solution, showing dark green; then add 3 drops of ammonia test solution and 6mL of water, shake well, and then show dark red colour in the light.

2. Fluorescence: Take a little of the product and immerse it in water or ethanol, the leachate shows blue fluorescence in daylight.

3. Thin-layer chromatography: take 1g of this product powder, add 10mL of ethanol, reflux on a water bath for 10min, cool, filtered, as a test solution. Another take Qinpi methylin and Qinpi ethylin control drugs, add ethanol to make each 1mL contains 5mg of mixed solution, as a control solution.

Pipette Take 3μL of each of the above two solutions, respectively, on the same silica gel G thin layer plate, toluene - ethyl acetate - ethanol - formic acid (3:4:2:1) as the unfolding agent, unfolding, remove, drying, and placed in the ultraviolet light (365nm) under the observation. The chromatogram of the test article showed fluorescent spots of the same colour in the corresponding positions with the chromatogram of the control article.

Ingredient of Esculin.

Aesculin (aesculin, hepcidin, fluoresces blue in aqueous solution at pH greater than 5.8), aes-culetin (aes-culetin, hepcidin, fluoresces blue in alkaline solution), frax-etin (fraxin), fraxin (fraxin), stylosin (stylosin), syringin and other coumarins. Essence class components. And contains tannins and alkaloids, etc.