Natural 4-aminobutyric Acid powder CAS 56-12-2

CAS:56-12-2

MF:C4H9NO2

MW:103.12

EINECS:200-258-6

Specification: 99% min 4-aminobutyric Acid Powder

Sample:4-aminobutyric Acid Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance:White

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

4-aminobutyric Acid Powder Usage and Synthesis.

Gamma-aminobutyric acid is a strong neuroinhibitory amino acid, with sedative, hypnotic, anticonvulsant and hypotensive physiological effects. It is InhibitoryNeurotransmitter, which can inhibit the activity of animals and reduce energy consumption.

Aminobutyric acid acts on γ-aminobutyric acid receptor in animal cells, γ-aminobutyric acid receptor is a chloride ion channel, the inhibitory or excitatory property of γ-aminobutyric acid is dependent on the concentration of chloride ions inside and outside of the cell membrane, γ-aminobutyric acid receptor activation leads to the opening of the chloride ion channel, which can increase the permeability of the cell membrane to chloride ions, so that the chloride ions flow into the neuronal cells, causing hyperpolarization of the cell membrane, inhibiting the neuronal cell element agitation, thus reducing the animal's locomotion.

Uses of 4-aminobutyric Acid.

Major inhibitory neurotransmitter in the brain; GABAA and GABAB receptor stimulant; increases Cl- conductance.

4-Aminobutyric acid has the effect of lowering blood lipids and is suitable for the treatment and prevention of various types of hepatic coma. It is used in the treatment of poliomyelitis, cerebral hemorrhage, and as an antidote for gas poisoning. Also used in biochemical research and organic synthesis.

It can reduce blood ammonia and promote brain metabolism. Used in the treatment of various types of hepatic coma, also used in post-stroke sequelae, cerebral arteriosclerosis, head trauma sequelae and uremia, gas poisoning and other coma caused by.

Preparation of 4-aminobutyric Acid.

Usually produced by pyrrolidone ring-opening. The lime is digested into lime milk with distilled water, pumped into the hydrolysis reactor, add pyrrolidone, heat up to 125 ~ 130 ℃, the reaction pressure is maintained at 0.29MPa, holding reaction more than 10 ~ 14h. After the end of the reaction, cool down to 30 ℃ discharge filtration, washed with distilled water.

Filtrate with ammonium bicarbonate until no calcium ions detected, and then add activated carbon at 80 ℃ insulation decolorization for 30min, 60 ℃ filtration, washed with distilled water, washings and filtrates combined, at 60 ℃ concentration under reduced pressure until the precipitation of crystals, add ethanol, cooling, filtration, drying, to get the finished product, the yield is more than 85%.

Product Method of Bulk 4-aminobutyric Acid Powder.

There are synthetic method and fermentation method.1. Synthetic method is produced by pyrrolidone ring-opening. Lime digested into lime milk with distilled water, pumped into the hydrolysis reactor, add pyrrolidone, heating to 125-130 ℃, the reaction pressure is maintained at 0.29MPa, insulation reaction more than 10-14h.

After the end of the reaction, cool down to 30 ℃ discharge filtration, washed with distilled water. Filtrate with ammonium bicarbonate until no calcium ions detected, then add activated carbon at 80 ℃ insulation decolorization for 30min, 60 ℃ filtration, washed with distilled water, washed with filtrate and combined at 60 ℃ concentrated under reduced pressure until the precipitation of crystals, add ethanol, cooling, filtration, drying, to get the finished product, the yield is 85% or more.2. Fermentation method using Escherichia coli as a strain.

Fermentation medium is bran hydrolysate, corn syrup, peptone, magnesium sulfate and sodium chloride. Soybean oil is used as defoamer, the dosage is about 0.1%, and the fermentation unit is about 100 enzyme units/ml fermentation solution.

In the extraction process, using the role of Escherichia coli decarboxylase, L-glutamic acid is converted into γ-ammonia tyrosine in aqueous solution can be dissociated into a cation of the characteristics of the strong acidic styrene-based cation exchange resin for ion exchange, ammonia elution, extraction, and then resin purification, concentration, crystallization, drying and then the finished product.