Natural Plant Epimedium Extract CAS 489-32-7

CAS:489-32-7

MF: C33H40O15

MW:676.66

EINECS:610-440-0

Specification: 99% min Epimedium Extract

Sample:Epimedium Extract Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance: Yellow Powder

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Epimedium Extract Usage and Synthesis.

Epimedium extract is processed from the dried stems and leaves of Epimedium brevicornum as raw materials, with more specifications of commercial extracts, usually with Epimedium glycosides or total flavonoids as standardised indexes.

Uses and functions of Epimedium Extract.

Epimedoside has the effect of increasing cardiovascular blood flow, promoting haematopoietic function, immune function and bone metabolism, tonifying the kidney and aphrodisiac, anti-aging, anti-tumour and so on.

1, the role of the endocrine: Epimedium glycosides can promote sexual function is due to hypersecretion of semen, seminal vesicles filled, stimulate the sensory nerves, and indirectly stimulate the libido and cause.

2, on the immune system function: kidney deficiency patients T cell number, gonorrhoea rate, antibodies, antigens and reticuloendothelial system phagocytosis function is low, and after Epimedium and other kidney tonic medicine treatment can be improved.

3, anti-aging effect: Epimedium can affect the aging mechanism from different aspects. For example, it can affect the cell transmission, prolong the growth period, regulate the immune and secretion system, and improve the metabolism of the body and the function of various organs.

Pharmacological Effect of Epimedium Extract.

1. Effects on sexual function Epimedium extract has a certain effect of promoting gonadal function. Flavonoid components such as Epimedium Glycoside has the effect of tonic kidney and aphrodisiac, 30 ~ 1000μg / l can promote the secretion of follicular granulosa cells estradiol, in 1000μg / l can promote the secretion of adrenal cortical cells corticosterone. At the same time, EPS can increase the level of gonadal hormones, mainly increase the level of FSH.

2. Improve the immune function of the body, Epimedium brevicornum 50% methanol extract can inhibit the transformation of lymphocytes. The total flavonoids of Epimedium can make the "Yang deficiency" mice form high cell function and antibody, and obviously enhance the human T cell immune function.EPS and EI have immunomodulatory effects.

3. Antioxidant EPS and EI have antioxidant activity can enhance the activity of antioxidant enzymes, reduce the role of free radical products.

4. Anti-aging effects of Epimedium extract can be affected by cell reproduction, prolong the growth period, regulate the immune and secretion system, improve the body metabolism and the function of various organs and anti-aging and prolong life.

5. On cardiovascular effects of Epimedium alcohol extract in the non-amino acid part can significantly increase the isolated rabbit heart coronary flow.

Decoction and alcoholic infusion have hypotensive effect. Epimedium glycoside can directly relax vascular smooth muscle, can dilate coronary artery, femoral artery and cerebral artery. The mechanism of action is to inhibit the effect of extracellular calcium ion inward flow in vascular smooth muscle.

Methods of analysis of Bulk Epimedium Extract

1. Thin layer identification method

Take Epimedium powder 0.5g or appropriate amount of extract, add 10ml of ethanol, warm immersion for 30 minutes, filtration, filtrate evaporation, residue with 1ml of ethanol to dissolve, that is, the test solution. Weigh the Epimedium Glycoside control, dissolved in methanol and diluted to 0.1mg/ml, that is, the control solution. Aspirate the test solution and the control solution of 10μl, respectively, point in the same sodium carboxymethyl cellulose as the binder of silica gel H thin layer plate, ethyl acetate - butanone - formic acid - water (10: 1: 1: 1: 1) as an unfolding agent, unfolding, take out, drying, placed in the ultraviolet lamp (365nm) to see the test chromatography, in the position corresponding to the chromatography of the control product showed the same dark red spots; sprayed with Aluminium trichloride test solution, and then placed in the ultraviolet lamp (365nm) inspection, the same orange-red fluorescent spots.

2. Determine the content of total flavonoids in Epimedium and its extracts by spectrophotometry Precisely weigh the control Epimedium glycoside, use methanol to form a standard solution of 10ug/ml. Precision weighing of the product powder appropriate amount (equivalent to about 1mg of Epimedium Chemicalbook Horny Goat Weed Glycosides), with methanol 25 ml ultrasonic 30 minutes, fixed in a 50 ml measuring flask, filtered through a 0.45 μm microporous filter membrane, that is, for the test solution. Take the test solution and the control solution respectively, use the reagent as the blank, determine the absorbance at 270nm, and the calculation is obtained.

3.HPLC method to determine the content of Icariin in Epimedium and its extract chromatographic conditions and system suitability test: ODS25uC18 (4mm×150mm) column; mobile phase: acetonitrile - water (30:70); detection wavelength: 270nm; flow rate: 0.8ml/min. Theoretical plate number is not less than 1500 in terms of Icariin.Preparation of control solution: Weigh precisely the control Epimedium Glycoside, with methanol to 0.1mg/ml solution.

Preparation of test solution: weigh the powder of this product (equivalent to about 1mg of Icariin), ultrasonic with 25ml of methanol for 30 minutes, and then set in a 50ml measuring flask, filtered with 0.45μm microporous membrane, that is, the test solution.