Supply Cholesterol Raw Materials powder Cholesterol

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Product details

(1) Participating in the formation of cell membranes.

Cholesterol is the raw material for the synthesis of bile acids, vitamin D, and steroid hormones.

(3) The total serum cholesterol of Chinese normal persons is about 182.5 ±4.3 mg %. High levels of cholesterol in the blood indicate that cholesterol metabolism may be impaired. Patients with coronary atherosclerosis often have high serum cholesterol levels. Therefore, the determination of serum cholesterol content in clinical laboratory will help to diagnose some diseases.

Uses of Cholesterol.

1. Cholesterol can be used as emulsifier, artificial bezoar, vitamin D, liquid crystal, raw materials for synthetic hormones, and also used as an analytical reference for biochemical research.

2. It can also be used for cephalin cholesterol flocculation test. Cholesterol is the main component of biofilms, and about 25% of total cephalin is cholesterol.

Pharmacological action of Cholesterol.

Product method of Bulk Cholesterol Powder.

Using animal brain tissue as raw material

Take fresh animal brain and spinal cord (remove fat and spinal cord membrane), ground, dried at 40-50℃ to obtain dried brain powder.

Brain (pig, cow, sheep) [40-50℃]→ Dried brain powder

Dry brain powder impregnated with 1.2 times the amount of acetone, continuously stirred, extracted for 4.5h, repeatedly extracted for 6 times, filtered, combined with the extraction solution, distilled to recover acetone, yellow solid was obtained. Add 10 times the amount of ethanol and heat reflux for 1h to obtain cholesterol ethanol solution, filter it, cool the filtrate at 0-5℃, let it stand, precipitate crystals, filter it, and obtain crude cholesterol crystals.

Dried brain powder [acetone]→ yellow solid [ethanol]→ cholesterol ethanol solution [0-5℃]→ crude cholesterol crystals

Preparation of refined cholesterol Take crude cholesterol crystals and add 5 times the amount of ethanol, add 5%-6% sulfuric acid, heat reflux for 8h, obtain hydrolysate, cool at 0-5℃, precipitate crystals, filter, obtain crystals, add ethanol to wash until neutral. The crystal washed to neutral was added with 10 times the amount of 95% ethanol and 3% activated carbon, heated for reflux decolorization for 1h, heat preservation and filtration, the filtrate cooled and crystallized at 0-5℃, repeated 3 times, filtered, collected crystals, pressed dry, dissipated the ethanol, and vacuum dried at 70-80℃ to obtain refined cholesterol.

Crude cholesterol crystals [ethanol, H2SO4]→[8h] hydrolysate [0-5℃]→ crystals [ethanol, activated carbon]→[1h] refined cholesterol.

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