Pharmaceutical intermediates Bulk L-Leucine Powder

CAS:61-90-5

MF:C6H13NO2

MW: 131.17

EINECS:200-522-0

Specification​: 99% min L-Leucine Powder

Sample:L-Leucine Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance:White Powder

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

L-Leucine Powder Usage and Synthesis.

L-Leucine is one of the eight essential amino acids, belonging to the aliphatic amino acids within the twenty protein amino acids, and L-isoleucine and L-valine, known as the three major branched-chain amino acids.

It is enantiomeric with D-Leucine, and is a white lustrous hexahedral crystal or white crystalline powder at room temperature.

Odourless, slightly bitter taste. In the presence of hydrocarbons, the performance is stable in aqueous solution of inorganic acids. Each gram is soluble in 40 ml of water and about 100 ml of acetic acid. Very slightly soluble in ethanol or ether, soluble in formic acid, dilute hydrochloric acid, alkaline hydroxide and carbonate solutions.

L-Leucine has the effect of promoting insulin secretion, can lower blood sugar; promote sleep, reduce sensitivity to pain, relieve migraine, ease agitation and tension, reduce the symptoms of chemical imbalance in the human body caused by alcohol, and help to control alcoholism; for the treatment of dizziness has a role in the treatment of dizziness, but also to promote the healing effect of the skin wounds and bones, so the doctor will usually recommend that post-surgery patients take Leucine supplements.

Often formulated into amino acid infusions and comprehensive amino acid preparations for therapeutic or health care applications; also used as an additive in food, cosmetics and feed. Plant growth promoter.

Uses and functions of L-Leucine.

Nutritional supplements; flavouring and aroma enhancers. Preparation of amino acid infusion and comprehensive amino acid preparation, hypoglycaemic agent plant growth promoter.

According to China GB2760-96 can be used as spices. Used in biochemical research, medicine for the treatment and diagnosis of idiopathic hyperglycemia in young children, and used in the treatment of anaemia, poisoning, myasthenia gravis, postpolio sequelae, neuritis and psychosis.

Amino acid drugs. Used as amino acid infusion and comprehensive amino acid preparations. Used in the diagnosis and treatment of idiopathic hyperglycaemia in young children and in the diagnosis and treatment of disorders of glucose metabolism, hepatic diseases accompanied by reduced bile secretion, anaemia, poisoning, muscular dystrophy, post-polio sequelae, neuritis and psychosis.

Diabetes mellitus, cerebral vascular sclerosis and renal disease with proteinuria and haematuria are contraindicated.

It is not suitable for patients with gastric and duodenal ulcer. It is an essential amino acid, with a requirement of 2.2g/d for adult men, which is necessary for the normal growth of infants and the maintenance of normal nitrogen balance in adults.

It can be used as nutritional supplements, amino acid infusion and comprehensive amino acid preparation, hypoglycaemic agent, plant growth promoter. It can be used as spice according to GB2760-86.

Used as pharmaceutical raw materials and food additives biochemical reagents, belongs to the human body must be amino acids, mainly as a nutritional supplement, with hypoglycemic and growth-promoting effect.

Characteristic of L-Leucine.

White lustrous hexahedral crystals or white crystalline powder. Slightly bitter taste (DL-Leucine is sweet). Sublimation at 145~148℃. Melting point 293~295°C (decomposition). In the presence of hydrocarbons, stable performance in aqueous inorganic acid solution. Each g dissolves in about 40 ml of water and about 100 ml of acetic acid

Per g is soluble in about 40 ml of water and about 100 ml of acetic acid. Slightly soluble in ethanol (0.07%), soluble in dilute hydrochloric acid and alkaline hydroxide and carbonate solutions. Insoluble in ether. It is an essential amino acid, and the requirement of adult men is 2.2g/d(151ben).

Necessary for the normal growth of infants and adults to maintain normal nitrogen balance. Natural products exist in the spleen, heart, etc., and in the form of protein in a variety of animal and plant tissues, and can be free after corruption and decomposition.

Production Method of Bulk L-Leucine Powder.

Blood powder as raw material

Blood powder [HCl (decomposition)] → [110 ℃, 24h] hydrolysate [deacidification] → [distillation under reduced pressure] deacidification solution [activated carbon (adsorption decolourisation)] → effluent [concentration] → [distillation under reduced pressure] concentrate [precipitation] → [o-xylene-4-sulphonic acid] precipitation [ammonia (free)] → [filtration] L-Leucine crude [activated carbon (decolourization)] → [70 ℃, 1h] filtrate [concentration of the crystalline] → [reduced pressure distillation] L-leucine crude [water (washing and drying) → L-leucine finished hydrolysis, deacidification in 1t hydrolysis tank by adding 6mol / L of HCl solution 500L, and put 100kg of animal blood powder, heating and holding 110-120 ° C reflux hydrolysis 24h, 70-80 ° C concentrated to paste under reduced pressure.

Then add 50L concentrated to paste, so repeated three times, cooled to room temperature, filtered and collected filtrate hydrolysate. Adsorption, decolourisation After diluting the hydrolysate with water by 1 times, the hydrolysate was flowed into a granular activated carbon column (30cm×180cm) at a flow rate of 0.5L/min until phenylalanine appeared in the effluent, and washed with deionised water at the same flow rate until the pH of the washed out solution was 4.0, and the effluent and the washed solution were combined to obtain the effluent solution.

Concentration, precipitation and freeing After the effluent was concentrated under reduced pressure to 1/3 of the original volume, 1/10 volume (V/V) of o-xylene-4-sulfonic acid was added with stirring to produce a leucine sulfonate precipitate, which was taken by filtration, washed twice with 2 times the volume of deionised water, and filtered and pressed to dryness to obtain leucine sulfonate.

Then stir with 2 times the volume of deionised water, then add 6 mol/L ammonia to neutralise to pH 6-8, keep warm and stir at 70-80 ℃ for 1h, so that the leucine from its sulfonate free. Cooling crystallisation, filtration to take the crystals, and then wash with 2 times the volume of deionised water for 2 times, filtration and pressure drying to get the leucine crude product. Refinement of leucine crude to 40 times the volume of deionized water heated to dissolve, add 0.5% activated carbon (5g / L) insulation at 70 ℃, stirring decolorization 1h, filtration of the filtrate, which will be concentrated to the original volume of 1/4, cooling crystals, filtration to take the crystals, and a small amount of water to wash, dry 70-80 ℃ drying, L-Leucine boutique.