Hemin Phosphate Raw Materials powder

CAS:16009-13-5

MF:C34H31ClFeN4O4-

MW:650.94

EINECS:240-140-1

Specification: 99% min Hemin powder

Sample:Hemin powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance: Black

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Hemin powder Usage and Synthesis.

Hemoglobin chloride (also known as iron porphyrin, hematoporphyrin, ferric hemoglobin chloride, hemoglobin) is extracted from pig and cow blood, which is recognized by modern medicine as a biological iron source with high absorption rate and good effect in the prevention and treatment of iron-deficiency anemia, with no ferric odor and no stimulation of the stomach and intestines. It is the preferred raw material for the production of iron-supplemented foods, medicines, health products and cosmetics.

Uses of Hemin powder.

Hemin chloride is both a substrate for HO-1 synthesis and a promoter of HO-1, which can induce HO-1 activity, improve plant resistance to oxidative stress and regulate plant metabolism.

It was found that heme chloride, as an HO-1 inducer, could significantly induce HO-1 expression by exogenous addition and alleviate the growth inhibition and oxidative stress induced by heavy metal stress by regulating the antioxidant system in crop roots.

Meanwhile, heme chloride could alleviate osmotic stress caused by salt stress. In addition, heme chloride promoted the accumulation of fresh weight and the growth of primary roots.

Biochemical research; used as blood test dye; used in the preparation of hematoporphyrin hydrochloride.

In the food industry, heme can replace the coloring agent nitrite and synthetic pigments in meat products; in the pharmaceutical industry, heme can be used as raw material for semi-synthetic bilirubin, and can be used for the preparation of anticancer drugs; in the clinic, heme can be made into heme iron supplements.

Nutritional supplements (iron fortification), extracted from pig's blood, recognized by modern medicine to prevent and treat iron deficiency anemia, high absorption rate, good effect of the downward bio-iron source, no iron fishy taste, does not stimulate the stomach and intestines. It is the preferred raw material for the production of natural iron supplement food, medicine, health care products and cosmetics.

Characteristic of Hemin powder.

Hemoglobin is the red blood pigment found in the blood and muscle of higher animals and is present in red blood cells, where it combines with proteins to form complex proteins known as hemoglobin (Hb) or myoglobin (Mb).Hb is a carrier for the transport of O2 and some CO2, and is a buffering substance that maintains the pH of the blood at a constant level.

Under certain conditions, hemoglobin can be separated from proteins. Hemoglobin is formed by complexing protoporphyrin IX with iron(II). Hemoglobin crystals are blue-black, insoluble in water, soluble in acidic acetone, alkaline aqueous solution, in solution easy to form polymers. Its molecule has a resonance structure, stable nature.

Hemoglobin chloride (Hemin) for the black-green amorphous or long needle-like crystals, insoluble in water, soluble in acidic acetone and alkaline water. Molecular formula C34H32ClFe(III)N4O4, molecular weight 651.96, mainly used for anti-anemia, anti-cancer drugs raw materials. 1930 H. Fischer (Germany) synthesized hemoglobin was awarded the Nobel Prize in Chemistry.

Nitrification of heme was carried out to free the iron ions therein and quantitatively generate an orange-red complex with an appropriate amount of o-phenanthroline in a buffer solution of pH 4.6, and the content of iron ions in heme was determined spectrophotometrically at (510±1) nm.

The results showed that the orange-red complex generated by iron ions with o-phenanthroline had maximum absorption at (510±1) nm and was in the range of 0.4-2.8 μg/ml, and the concentration and absorption were in accordance with Beer's law, with the correlation coefficient of r=0.9999, and the average recovery of the method was X=98.87% with RSD=0.58%.

Product Method of Bulk Hemin powder.

Alkaloid-organic solvent method

Take 60L of methanol and 1kg of diethylamine added to the reaction tank, stirred well, add 5kg of fresh bovine blood powder, extracted at 45℃ with stirring for 1h, cooled, filtered, filtrate concentrated to 5L, add 10L of glacial acetic acid, 200g of strontium chloride, heat distillation to remove residual methanol, warmed up to 100-102 ℃ reaction for 1h, cooled, filtered, collected hemoglobin chloride crystals.

Hemoglobin chloride crystals were washed with glacial acetic acid, water, acetone in turn, drying, to produce 85g of hemoglobin chloride.

Bovine blood powder [methanol, diethylamine] → [45 ℃, 1h] [filtration] → filtrate [ice Hac, SrCl2] → [100-102 ℃, 1h] [filtration, washing, drying] → hemoglobin chloride Take 60L of methanol and 5kg spray-dried bovine erythrocytes to add into the reaction tank, stir well, extracted at 40 ℃ for 30min, cooling, filtration, filtrate concentrated to 5L, add glacial acetic acid 10L, add glacial acetic acid, 100g of sodium chloride, add 2L of water to dissolve, heating distillation to remove residual methanol, heating to 100 ℃ reaction 1h, cooling, filtration, collection of hemoglobin chloride crystals. Hemoglobin chloride crystals were washed with glacial acetic acid and water in turn, and dried to produce 65g of hemoglobin chloride.

Bovine erythrocytes [methanol] → [40 ° C, 30min] [filtration] → filtrate [ice Hac, NaCl, H2O] → [100 ° C, 1h] [filtration, washing, drying] → heme chloride hemoglobin hemoglobin preparation take fresh blood powder 300kg, add methanol 1200L, add 30kg of dichloroacetic acid, stirring uniformly, stirring with 45 ° C extraction of 1h, cooling, filtration, filtrate concentration To dry, get hemoglobin 45kg.

Fresh blood powder [methanol, dichloroacetic acid] → [45 ℃, 1h] [filtration, concentration] → hemoglobin the preparation of heme chlorinated hemoglobin take hemoglobin 45kg, add 100L of glacial acetic acid containing 2kg of NaCl, boil and reflux extraction for 1h, cooling, filtration, to get chlorinated hemoglobin, in turn, with glacial acetic acid, water washing, drying, to get chlorinated hemoglobin 4.8kg.

Hemoglobin [NaCl and glacial acetic acid mixed solvent] → [boiling 1h] [washing, drying] → hemoglobin chloride.