Raw Materials Procyanidin Powder

CAS:4852-22-6

MF:C30H26O13

MW:594.52

EINECS:1806241-263-5

Specification: 99% min Procyanidin Powder

Sample:Procyanidin Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance:Brown Red

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Procyanidin Powder Usage and Synthesis.

Proanthocyanidins are a large group of polypolyphenols with structural similarity to anthocyanins that are widely found in plants and are formed by condensation of flavan-3-ol monomers.

Structurally, proanthocyanidins are formed by the combination of varying amounts of catechins (catechin) or epicatechins (epicatechin).

The simplest proanthocyanidins are dimers formed by catechin, or epicatechin, or catechin and epicatechin, but there are also trimers, tetramers, etc. up to decamers.

According to the size of the degree of polymerisation, two to five polymers are usually called oligomeric proanthocyanidins (OPC for short), and more than five polymers are called polymeric proanthocyanidins (PPC for short).

Proanthocyanidins have very strong antioxidant and other activities, and have been widely used in food, drugs, cosmetics and other fields.

Proanthocyanidins extracted from blueberry leaves can resist hepatitis C virus.

Uses of Procyanidin.

Proanthocyanidins (Grape Seed Extract) is a new type of highly effective antioxidant, the most potent free radical scavenger found so far, with very strong in vivo activity.

It is used to improve blood circulation, treat diabetic retinopathy, reduce oedema and inhibit varicose veins, prevent complications after cataract surgery in diabetic patients, eliminate oedema and moisturise the skin.

The combination of anthocyanin and vitamin C can make cholesterol decomposition, as a cardioprotective agent, etc.. Used for content determination/identification/pharmacological experiments, etc.

Chemical reaction of  Bulk Procyanidin Powder.

Proanthocyanidins are a class of polyphenolic compounds synthesised via the secondary metabolic pathway of plant flavonoids and are oligo- or polymers of flavan-3-ols such as catechins and epicatechins. The biosynthesis all starts with phenylalanine.

Hypotheses on the mechanism of their polymerisation include that the polymerisation of proanthocyanidins occurs in the central macrovesicle of the plant cell, and that the precursors of the extension unit in the condensation process may be colourless anthocyanins, flavan-3-ols and anthocyanidins.

The condensing enzymes that catalyse the polymerisation reaction may include: plant polyphenol oxidase, plant laccase and plant peroxidase.

Preparation method of Procyanidin.

1.1 Extraction At present, the commonly used process is the first defatting methods including pressing, solvent method, supercritical CO2 extraction, of which, the supercritical CO2 extraction method is the best, not only the oil and fat extraction rate is high, but also the destructive effect on the proanthocyanidins is minimised, and the quality is better.

1.2 Separation and purification Proanthocyanidin monomer substances are usually separated by column chromatography, in which polyamide, SephadexLH-20 and ToyopealHW-40 are the most effective packing materials.

For compounds that are more difficult to separate or require smaller amounts, they can be prepared by semi-preparative reversed-phase high-performance liquid chromatography (RP-HPLC) and normal-phase high-performance liquid chromatography (NP-HPLC).

The number of isomers of proanthocyanidins increases geometrically with increasing degree of polymerisation, and it is very difficult to isolate and purify such macromolecular monomeric substances. For polymers, they can be segmented by molecular weight (degree of polymerisation) size.

At present, established classification methods include solvent precipitation and various chromatographic methods, such as thin-layer chromatography, normal-phase high-performance liquid chromatography, gel exclusion chromatography, counter-current chromatography and so on.