Trehalose Raw Materials CAS 99-20-7

CAS:99-20-7

MF:C12H22O11

MW:342.3

EINECS:202-739-6

Specification: 99% min Trehalose Powder

Sample:Trehalose Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance:White

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Trehalose Powder Usage and Synthesis.

Alginose has the special function of preserving biological vitality, which can effectively protect the cell membrane and protein structure, so that the organism can keep the cell moist under abnormal conditions, such as high temperature dehydration (drying, high osmotic pressure) and freezing, preventing the loss of nutrients and cell damage due to the loss of water in the cell.

Moreover, in vitro alginate also has the property of stabilizing biofilm and protein structure, internationally it is used to protect genetically engineered enzymes, various viruses, vaccines, antibodies, protein factors, nucleic acids, etc. At the same time, as a stabilizer and protector of biologically active substances, it has broad application prospects in the fields of food, health care, cosmetics, pharmaceuticals, molecular biology and agriculture.

Uses of Trehalose.

Glyceryl laurate is an antibacterial, antiviral and antifungal substance that destroys the outer membrane of microorganisms, thus killing them. Glyceryl laurate prevents infectious diseases such as influenza and protects against harmful bacteria in infants.

Coconut oil contains lauric acid, which is one of the raw materials used in the production of glyceryl laurate. Glycerol laurate is also effective in helping female monkeys avoid infection with the monkey immunodeficiency virus (SIV), which is expected to provide a new approach to preventing HIV infection in humans.

Pharmacological effect of Trehalose.

Alginate has a special protective effect on biomolecules, which enables many organisms to remain active under abnormal conditions, such as high temperature, dehydration and freezing. The presence of bound water on the surface of biological membranes plays an important role in stabilizing and maintaining the integrity of the membrane.

Under normal conditions, a large amount of bound water exists on the membrane surface, for example, in PC liposomes, usually 10-15 water molecules are bound to each phospholipid head. The presence of bound water reduces the surface energy of the membrane, preventing the first step of membrane fusion, which is the close contact between the membrane and the membrane, from being realized. However, once the bound water is lost, the membrane structure will undergo a series of changes, such as an increase in the phospholipid phase transition temperature, separation of phospholipid lateral phases, defects in membrane lipids and the formation of non-bilayer phases, which will lead to an increase in the permeability of the membrane and even the fusion of the membrane.

As alginate has many hydroxyl groups, it can replace water and phospholipid head hydrogen bonding, thus replacing the lost bound water and maintaining the "hydrated" state of the biological membrane surface. Human skin cells are also a typical biofilm. Alginate protects the skin by lowering the phase transition temperature so that the membrane lipids are still in the liquid crystal state under dehydration conditions. Experiments have shown that the protection of biofilm by alginate is realized by anti-fusion, leakage and other pathways.

Proteins, especially enzymes, are easily inactivated during the drying process, for example, phosphofructose kinase (Phosphofructosekinase, PFK) is a tetramer, which can dissociate into inactive dimers during the drying process. If a certain amount of alginate is added before drying, PFK is completely stabilized and remains active even after extreme drying.

Camilo et al. placed ECOR Ⅰ, Bg Ⅲ, Pst Ⅰ and Hind Ⅲ, T4 DNA ligase etc. in alginate-containing buffer for a certain period of time, and then ventilated and dried at 37 ℃. After drying, the enzymes were stored at 70 ℃ for 35d and could still cut and ligate the DNA accurately, whereas the activity of the enzymes without alginate was completely lost.

Product Method of Bulk Trehalose Powder.

1.In nature, it exists in the free state in mushrooms and other fungi, seaweed, shrimp and baker's yeast, brewer's yeast. Production method: Using starch as raw material, liquefied, fermented with heat-resistant α-amylase, saccharified, and then obtained by the action of isomerized amylase.

2. Produced by fermentation method, the fermentation broth is passed through anion exchange resin (amberliteCG1201), and the purity of seaweed sugar can be obtained as 98.4%.