Supply Dl-Dithiothreitol Raw Materials powder

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Dl-Dithiothreitol of Usage and Synthesis.

Dithiothreitol (DTT) is a small molecule organic reducing agent with the chemical formula C4H10O2S2, which is mainly used in the field of biotechnology as a protein cracking reagent. At present, the most widely used synthesis method of dithiothreitol is based on threitol as raw material, which is oxidized with potassium permanganate, then gets thiothreitol under the action of thioacetic acid, and finally gets dithiothreitol by hydrolysis of the thiointermediate.

Uses of Dl-Dithiothreitol.

Used for An excellent reagent for keeping the SH group in the reducing state; Quantitative reduction of disulfide; Before SDS-PAGE, DTT can effectively reduce protein disulfide bond in sample buffer. DTT can also be used to reduce the disulfide bridge of the crosslinker N,N '-bis (acrylyl) cystamine to decompose the polyacrylamide gel matrix. Used to remove disulfide connections in protein sequence analysis; To investigate increased and dynamic reduction reactions; Blocking the oxidation that occurs when cholecystokinin is extracted from brain and duodenal tissue.

Effect of Dl-Dithiothreitol.

Dithiothreitol has antioxidant effect, it can protect the reducing groups on the enzyme molecules, maintain the reducing environment, and stabilize the activity of the enzyme. DTT also contains some RNase inhibitors, which can inhibit the activity of RNase. It has a similar effect to mercaptoethanol (dimercaptoethanol), but DTT has a much less irritating odor and is much less toxic than mercaptoethanol. Moreover, when the concentration of DTT is 7 times lower than that of mercaptoethanol, the two effects are similar. But DTT prices are slightly higher. Cleavage and sequence analysis of protein disulfide bonds used as protein Euclidean protection agents.

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