Lysozyme Raw Materials Lysozyme Powder

CAS:12650-88-3

MF:C125H196N40O36S2

MW:2899.27014

EINECS:235-747-3

Specification: 99% min Lysozyme Powder

Sample:Lysozyme Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance:White

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Lysozyme Powder Usage and Synthesis.

Application in medicine: It is mainly used for many mucous membrane inflammation in Pentacenter, such as paranasal sinusitis, chronic rhinitis, acute and chronic pharyngolaryngitis, lichen planus, oral ulcers, oozing otitis media, and dermatology, such as herpes zoster, warts, chicken pox, etc. It is also used for asthmatic bronchitis, ulcerative colitis, mumps, etc. It is also used in asthmatic bronchitis, ulcerative colitis, mumps, etc. It is more effective when used with antibacterial drugs.

Application in biotechnology: Lysozyme has the function of destroying the structure of bacterial cell wall, and G+ bacteria can be treated with lysozyme to get protoplasmic body after destroying the cell wall, so it is widely used in biotechnology, and it has become an indispensable instrumental enzyme for the operation of cell fusion in genetic engineering and cell engineering.

In aquatic products and meat products in the application: for lysozyme non-toxic, harmless properties, and at the same time on the decomposition of G + cell wall, applied to aquatic products and meat products, in order to ensure that the original nutrients of food without loss of the premise of aquatic products and meat products can play a role in preservation and anticorrosion; the use of lysozyme and sodium chloride and nitrite joint application, can extend the shelf life of meat products, and its anticorrosion The effect is better than when used alone; test with 0.05% of lysozyme and phytic acid polymerization phosphate, glycine, etc. used in conjunction, as a preservative and preservative can be seafood and aquatic products to extend the storage period.

Application in other foods: Cream cake is one of the foods that are easy to be corrupted and spoiled, adding lysozyme in pastry can prevent the phenomenon of corruption and deterioration caused by microorganisms from occurring; applying lysozyme in the preservation of food, and its effect is also extremely obvious.

At the same time, according to the lysozyme non-toxic, harmless and health effects, anti-infective and enhance the effectiveness of antibiotics, as well as to promote blood coagulation and hemostasis and tissue regeneration, now some people try to enhance the health effects of food as an additive in food.

Uses and Function of Lysozyme.

Use: Biochemical research. It is an alkaline enzyme that hydrolyzes mucopolysaccharides in pathogenic bacteria. Mainly by destroying the β-1,4 glycosidic bond between N-acetyl cytosolic acid and N-acetyl aminoglucose in the cell wall, it decomposes the insoluble mucopolysaccharides of the cell wall into soluble glycopeptides, leading to the rupture of the cell wall and the escape of the contents and lysis of bacteria.

Lysozyme can also be combined directly with negatively charged viral proteins to form complex salts with DNA, RNA, and de-coordinated proteins to inactivate viruses. It can decompose Gram-positive bacteria such as Micrococcus solani, Bacillus megaterium, and yellow octococcus.

Mechanism of action of Lysozyme.

Lysozyme has a good decomposition effect on Gram-positive bacteria, aerosol spore-forming bacteria, Bacillus subtilis, radiation-resistant micrococcus, and also has a certain degree of solubilization effect on Gram-negative bacteria such as Escherichia coli, common variola and Vibrio parahaemolyticus. Lysozyme has the special function of decomposing peptidoglycan in bacterial cell wall.

Bacterial cell wall consists of cytoplasm, cytoplasm is composed of N-acetylglucosamine and N-acetylcytidylic acid alternately composed of polymers, cytidylic acid residues can be connected to peptides, called peptidoglycan.

Peptidoglycan exists in the form of a straight chain, and peptidoglycan chains in close proximity to each other can be partially interconnected by peptidoglycan chains, thus forming a three-dimensional structure.

The active center of the enzyme is aspartic acid and glutamic acid, lysozyme through its peptide bond in the 35th position of glutamic acid and 52nd position of aspartic acid constitutes the active part of hydrolysis to destroy the microbial cell wall peptidoglycan molecules composed of N-acetyl cytosolic acid (NAM) and N-acetyl glucose ammonia (NAG) β-1,4 glycosidic bond between the cells due to osmotic imbalance caused by the rupture, which led to the bacterial body The cell wall is lysed and the bacteria (especially cocci) are killed.

Product Methods of  Bulk Lysozyme Powder.

Egg white is used as raw material, adsorbed with weakly acidic cation exchange resin 732 under the condition of pH 6.5, then eluted with ammonium sulfate, and freeze-dried after dialysis to get the product.