Pharmaceutical 98% Sesamin CAS 607-80-7

CAS:607-80-7

MF: C20H18O6

MW:1354.35

EINECS:/

Specification: 99% min Sesamin

Sample: Sesamin Powder

Packaging:1kg/bag, 25kg/drum

Brand: Henrikang

Appearance: White Powder

Storage: Cool Dry Place

Shelf Life: 2 Years

Test Method: HPLC

Sesamin Usage and Synthesis.

Sesquiterpenes are known to regulate blood pressure and blood lipids, protect and strengthen liver function, antioxidant and tumour inhibitor.

Content determination of Sesamin.

A method for determining the content of sesquiterpenes in sesame seeds, comprising the following steps:

(1) Preparing sesquiterpene standard solution: weighing 10 mg of sesquiterpene standard, adding anhydrous methanol to dissolve and dilute to 50 mL, and then filtering with 0.45 μm filtration membrane to obtain 0.2 mg/mL of sesquiterpene standard solution;

(2) Drawing the standard curve of sesamin: take 20, 10, 5, 4, 2, 0.8mL of the standard solution of sesamin obtained in step (1), add 0, 10, 15, 16, 18, 19.2mL of anhydrous methanol, respectively, and shake well to form a methanolic solution of sesamin standard with gradient concentration of 200, 100, 50, 40, 20, 8ug/mL, respectively, and then use liquid chromatography to perform chromatography on the above solutions. Chromatographic analysis of the above solutions, respectively, according to the results of the standard curve drawn sesquiterpenes (sesquiterpene standard solution of the liquid chromatogram refer to Figure 1), and get the sesquiterpene content and the peak area of the linear relationship between the equation: A = 587.525675x-4.531582, Chemicalbook where A is the peak area, x for the sesquiterpene content;

(3) Preparation of the sample solution to be tested: using Yuzhi 11 sesame seeds as the sample to be tested, the sesame seeds to be tested were crushed with a universal pulveriser for 30s, and then sieved through a 0.35 μm sieve, and the resulting sesame seed powder sample was taken as 0.2 g, and ethanol at a concentration of 100% was added to 5 mL;

(4) ultrasonic treatment and filtration: the mixed solution obtained in step (3) is ultrasonicated for 20 to 50 min at 30 to 60 ℃, and then the ultrasonicated solution is filtered with a 0.45 μm filtration membrane; the ultrasonic treatment is performed under the following conditions: using a numerically-controlled ultrasonic cleaner with an ultrasonic frequency of 40 KHZ and a model number of KQ3200DE, and the ultrasonic treatment is carried out under the conditions of 70 to 100 W The ultrasonic treatment is carried out under the ultrasonic power of 70-100W.

(5) Detection: take 9 μl of the filtrate obtained in step (4), use the liquid chromatograph to sample the filtrate for detection, and calculate the content of sesquiterpenes in the sesquiterpenes to be tested according to the standard curve of sesquiterpenes and the obtained linear equation.

Pharmacological Effect of Sesamin.

Sesquiterpenes have inhibitory effects on influenza virus, Sendai virus and Mycobacterium tuberculosis. Used in agriculture as a synergist for pyrethroids. Clinically observed to be effective against bronchitis. Used as antiviral, fungicide, antioxidant, insecticide synergist, treatment of bronchitis.

Preparation of Bulk Sesamin.

Measure 1L of sesame oil foot (965.8g), add 4L of anhydrous ethanol, stirring at 30 ℃ for 5h, static stratification, take the upper layer of liquid, rotary evaporation to get the sesame pigment enrichment; to sesame pigment enrichment to add anhydrous methanol, sesame pigment enrichment with anhydrous methanol material-liquid ratio of 1:4 (kg/L), at 55 ℃ under the extraction of 3h, get the extract; to the extraction of the liquid to add activated carbon. Activated carbon added to the extraction solution, the amount of 1% of the mass of the extraction solution, stirring for 0.5h, and then filtered to obtain the sesquiterpene extract; the sesquiterpene extract slowly cooled down to -10 ℃, so that the sesquiterpene crystallisation, crystallisation of 2h, filtration of sesquiterpene crude; to the sesquiterpene crude added to the petroleum ether, sesquiterpene crude and the petroleum ether of the material-liquid ratio of 1:1 (kg/L), 35 ℃, closed mixing for 0.5h, filtration Sesquiterpene crude product; sesquiterpene crude product dissolved in anhydrous ethanol, sesquiterpene crude product and anhydrous ethanol material-liquid ratio of 1:30 (kg / L), at -5 ℃ under the crystallisation and purification, the time to maintain 3 hours, under this condition after two times crystallisation, vacuum drying to obtain sesquiterpene products 2.16 g, sesquiterpene extraction rate of 89.1%, the purity of the resulting sesquiterpene products is 92.1%.